Anticorps Monoclonal anti-SPINT1/HAI-1

SPINT1/HAI-1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H2D8

N° de cat : 67031-1-PBS

Synonymes

HAI 1, HAI-1, SPINT1, 2H2D8, HAI

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Galerie de données de validation

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  WB analysis of human placenta using 67031-1-Ig (same clone as 67031-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67031-1-Ig (HAI-1 antibody) at dilution of 1:900 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67031-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67031-1-Ig (same clone as 67031-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using HAI-1 antibody (67031-1-Ig, Clone: 2H2D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67031-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67031-1-PBS cible SPINT1/HAI-1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SPINT1/HAI-1 Protéine recombinante Ag26733
• Nom complet: serine peptidase inhibitor, Kunitz type 1
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC004140
• Symbole du gène: HAI-1
• Identification du gène (NCBI): 6692
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is also named as SPINT1 and Kunitz-type protease inhibitor 1. HAI-1 is critical to control matriptase proteolytic activity (PMID: 25310042). HAI-1 inhibits matriptase activity through forming complex with activated matriptase (PMID: 30370662). matriptase, HAI‐1 also binds to and inhibits a variety of serine proteases including of hepsin, plasmin, prostasin, TMPRSS13 and HAT. HAI-1 as a cell-autonomous tumor suppressor that negatively regulates the HGF pathway (PMID: 25925948). HAI-1 is inhibitor of HGFAC (PMID: 9045658). HAI-1 inhibits serine protease activity of ST14/matriptase in vitro (PMID: 28710277). HAI-1 inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PMID: 20977675). HAI-1 expression is reduced in many cancer types, which is implicated in the progression of cancer and is associated with a worse prognosis in cancer patients (PMID: 30370662).

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