Anticorps Polyclonal de lapin anti-HAP1

HAP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25133-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of fetal human brain using 25133-1-AP (same clone as 25133-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25133-1-AP (HAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 25133-1-AP (same clone as 25133-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25133-1-AP (HAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25133-1-AP (same clone as 25133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25133-1-AP (HAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25133-1-AP (same clone as 25133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25133-1-AP (HAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 25133-1-AP (same clone as 25133-1-PBS)

  Immunofluorescent analysis of SH-SY5Y cells using 25133-1-AP (HAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25133-1-PBS cible HAP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HAP1 Protéine recombinante Ag17705
• Nom complet: huntingtin-associated protein 1
• Masse moléculaire calculée: 671 aa, 76 kDa
• Poids moléculaire observé: 75-80 kDa
• Numéro d’acquisition GenBank: BC156728
• Symbole du gène: HAP1
• Identification du gène (NCBI): 9001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HAP1 (Huntingtin-associated protein 1) was originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT. HTT is expressed ubiquitously, while HAP1 is expressed predominantly in the central nervous system (CNS). Both HTT and HAP1 participate in intracellular trafficking. HAP1 is involved in the vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation (PMID: 19262167).

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