Anticorps Polyclonal de lapin anti-HARS

• Phare
• Validé par KD/KO

HARS Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 16375-1-AP

Synonymes

Histidyl-tRNA synthetase, Histidine--tRNA ligase, cytoplasmic, HisRS, HARS1, EC:6.1.1.21

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Galerie de données de validation

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  WB analysis of mouse liver using 16375-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16375-1-AP

  WB result of HARS antibody (16375-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HARS transfected HeLa cells.

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  WB analysis of K-562 using 16375-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16375-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 16375-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16375-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16375-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 16375-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16375-1-AP (HARS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 16375-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16375-1-AP (HARS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, cellules K-562, tissu cardiaque de souris, tissu cérébral de souris, tissu pulmonaire de souris, tissu rénal humain
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

16375-1-AP cible HARS dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HARS Protéine recombinante Ag9333
• Nom complet: histidyl-tRNA synthetase
• Masse moléculaire calculée: 509 aa, 57 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC011807
• Symbole du gène: HARS
• Identification du gène (NCBI): 3035
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. The D364Y (Asp364-to-Tyr) mutation of HARS1 inhibits morphological differentiation of neuronal cells (PMID: 35380399).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics.; Authors - Maria C Passarelli
human	WB	Mol Cell; A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation; Authors - Raja H Ali
mouse	WB	Sci Rep; NRF2 activation ameliorates blood-brain barrier injury after cerebral ischemic stroke by regulating ferroptosis and inflammation; Authors - Wei Fan
	WB	Front Pharmacol; S1PR3, as a Core Protein Related to Ischemic Stroke, is Involved in the Regulation of Blood-Brain Barrier Damage.; Authors - Xuehui Fan
Mouse	WB	BMC Med Genomics; Analysis of ferroptosis-related genes in cerebral ischemic stroke via immune infiltration and single-cell RNA-sequencing; Authors - Wei Fan
mouse	WB	Neuroscience; MyD88 inhibition attenuates cerebral ischemia-reperfusion injury by regulating the inflammatory response and reducing blood-brain barrier damage; Authors - Fangchao Jiang

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