Anticorps Monoclonal anti-HARS

HARS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B7G2

N° de cat : 67662-1-PBS

Synonymes

FLJ20491, HARS, HisRS, Histidine tRNA ligase, histidyl tRNA synthetase, HRS

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Galerie de données de validation

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  WB analysis using 67662-1-Ig (same clone as 67662-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67662-1-Ig (HARS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67662-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67662-1-Ig (same clone as 67662-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HARS antibody (67662-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67662-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67662-1-PBS cible HARS dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HARS Protéine recombinante Ag9224
• Nom complet: histidyl-tRNA synthetase
• Masse moléculaire calculée: 509 aa, 57 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC011807
• Symbole du gène: HARS
• Identification du gène (NCBI): 3035
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

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