Anticorps Polyclonal de lapin anti-HARS2

• Phare
• Validé par KD/KO

HARS2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11301-1-AP

Synonymes

HARS2, HARSL, HARSR, HisRS, Histidine tRNA ligase, Histidine tRNA ligase like, HO3

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Galerie de données de validation

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  WB analysis of MCF-7 using 11301-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11301-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11301-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11301-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11301-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11301-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11301-1-AP

  IP Result of anti-HARS2 (IP:11301-1-AP, 4ug; Detection:11301-1-AP 1:500) with HEK-293 cells lysate 3000ug.

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  IHC staining of human breast cancer using 11301-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11301-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11301-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11301-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11301-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HEK-293, cellules MCF-7, tissu cardiaque humain, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

11301-1-AP cible HARS2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HARS2 Protéine recombinante Ag1827
• Nom complet: histidyl-tRNA synthetase 2, mitochondrial (putative)
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC014982
• Symbole du gène: HARS2
• Identification du gène (NCBI): 23438
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HARS2, also named as HARSL, HARSR, HO3 and HisRS, belongs to the class-II aminoacyl-tRNA synthetase family. It is an enzyme that catalyzes the esterification of histidine to its cognate tRNA as an early step in protein biosynthesis. When overexpressed in HIV-1-producing cells, HARS2 was incorporated into wild-type virions but not in ones containing the dilysine-mutated variant of matrix(MA) protein . HO3, through its recognition of MA, plays a role in the life cycle of HIV-1. (PMID: 9445076 ,7755634 ). 1-33aa of HARS2 is transit peptide and this part will be cut in some form(~52kd) of HARS2. This antibody can bind both HARSs for the close sequences.

Publications

Species	Application	Title
human	WB	J Biol Chem; Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation.; Authors - Shasha Gong
mouse	WB	Front Cell Neurosci; Disruption of Hars2 in Cochlear Hair Cells Causes Progressive Mitochondrial Dysfunction and Hearing Loss in Mice.; Authors - Pengcheng Xu ; KO Validated
mouse	WB	Cells; CLPP Depletion Causes Diplotene Arrest; Underlying Testis Mitochondrial Dysfunction Occurs with Accumulation of Perrault Proteins ERAL1, PEO1, and HARS2; Authors - Jana Key