Anticorps Recombinant de lapin anti-HAS2

• Phare
• Validé par KD/KO

HAS2 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240061G4

N° de cat : 83204-2-PBS

Synonymes

240061G4, EC:2.4.1.212, HA synthase 2, hyaluronan synthase 2, Hyaluronate synthase 2

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Galerie de données de validation

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  WB analysis using 83204-2-RR (same clone as 83204-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83204-2-RR (HAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83204-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 83204-2-RR (same clone as 83204-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 83204-2-RR (HAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83204-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 83204-2-RR (same clone as 83204-2-PBS)

  WB result of HAS2 antibody (83204-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAS2 transfected HEK-293 cells. This data was developed using the same antibody clone with 83204-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00153-1

  Cytometric bead array standard curve of MP00153-1, HAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83204-2-PBS. Detection antibody: 83204-1-PBS. Standard: Ag34945. Range: 0.625-20 ng/mL.

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  Cytometric bead array standard curve of MP00153-3

  Cytometric bead array standard curve of MP00153-3, HAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83204-2-PBS. Detection antibody: 83204-3-PBS. Standard: Ag34945. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83204-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83204-2-PBS against Human HAS2 was performed. The affinity constant is 0.516 nM.

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  Affinity and Kinetic Characterization of 83204-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83204-2-RR against Human HAS2 were performed. The affinity constant is 1.37 nM.

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Informations sur le produit

83204-2-PBS cible HAS2 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HAS2 Protéine recombinante Ag34945
• Nom complet: hyaluronan synthase 2
• Masse moléculaire calculée: 552 aa, 64 kDa
• Poids moléculaire observé: 63-67 kDa
• Numéro d’acquisition GenBank: BC069353
• Symbole du gène: HAS2
• Identification du gène (NCBI): 3037
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RIT2 (Hyaluronan synthase 2) is a member of the gene family encoding the hyaluronan synthase 2, which can generate high-molecular-weight hyaluronan (HMW-HA) (PMID: 35069543). HAS2 is the most critical synthase in producing hyaluronan and is well known for its involvement in cancer growth, metabolism and metastasis (PMID: 36386154). HAS2 is involved in cellular acquired resistance to drug therapy in BrCa. HAS2 expression was decreased in the endocrine-resistant ER+BrCa cells.

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