Anticorps Recombinant de lapin anti-HAS2

• Phare
• Validé par KD/KO

HAS2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240061G4

N° de cat : 83204-2-RR

Synonymes

Hyaluronic acid synthase 2, Hyaluronate synthase 2, hyaluronan synthase 2, HA synthase 2, EC:2.4.1.212

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Galerie de données de validation

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  WB analysis of HEK-293 using 83204-2-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 83204-2-RR (HAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83204-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83204-2-RR (HAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 83204-2-RR

  WB result of HAS2 antibody (83204-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAS2 transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 83204-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83204-2-RR against Human HAS2 were performed. The affinity constant is 1.37 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HCT 116, cellules HT-29, cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83204-2-RR cible HAS2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HAS2 Protéine recombinante Ag34945
• Nom complet: hyaluronan synthase 2
• Masse moléculaire calculée: 552 aa, 64 kDa
• Poids moléculaire observé: 63-67 kDa
• Numéro d’acquisition GenBank: BC069353
• Symbole du gène: HAS2
• Identification du gène (NCBI): 3037
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RIT2 (Hyaluronan synthase 2) is a member of the gene family encoding the hyaluronan synthase 2, which can generate high-molecular-weight hyaluronan (HMW-HA) (PMID: 35069543). HAS2 is the most critical synthase in producing hyaluronan and is well known for its involvement in cancer growth, metabolism and metastasis (PMID: 36386154). HAS2 is involved in cellular acquired resistance to drug therapy in BrCa. HAS2 expression was decreased in the endocrine-resistant ER+BrCa cells.

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