Anticorps Monoclonal anti-HAT1

• Phare
• Validé par KD/KO

HAT1 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A11F6

N° de cat : 67971-1-PBS

Synonymes

1A11F6, EC:2.3.1.48, KAT1

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Galerie de données de validation

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  WB analysis using 67971-1-Ig (same clone as 67971-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67971-1-Ig (HAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67971-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67971-1-Ig (same clone as 67971-1-PBS)

  WB result of HAT1 antibody (67971-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67971-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 67971-1-Ig (same clone as 67971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67971-1-Ig (HAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67971-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 67971-1-Ig (same clone as 67971-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HAT1 (67971-1-Ig, Clone:1A11F6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67971-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67971-1-PBS cible HAT1 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HAT1 Protéine recombinante Ag1952
• Nom complet: histone acetyltransferase 1
• Masse moléculaire calculée: 419 aa, 50 kDa
• Poids moléculaire observé: 45-46 kDa
• Numéro d’acquisition GenBank: BC018682
• Symbole du gène: HAT1
• Identification du gène (NCBI): 8520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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