Anticorps Polyclonal de lapin anti-HAUS1

HAUS1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11094-2-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of human skeletal muscle using 11094-2-AP (same clone as 11094-2-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11094-2-AP (same clone as 11094-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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  WB analysis using 11094-2-AP (same clone as 11094-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11094-2-AP (same clone as 11094-2-PBS)

  IP result of anti-HAUS1 (IP:11094-2-AP, 4ug; Detection:11094-2-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11094-2-AP (same clone as 11094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11094-2-AP (same clone as 11094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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Informations sur le produit

11094-2-PBS cible HAUS1 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HAUS1 Protéine recombinante Ag1569
• Nom complet: coiled-coil domain containing 5 (spindle associated)
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC014003
• Symbole du gène: HAUS1
• Identification du gène (NCBI): 115106
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HAUS1 is a subunit of the human augmin complex that is involved in microtubule generation and mitotic spindle formation (PMID:27036161). In addition, HAUS1 may play a major role in centrosome integrity and completion of cytokinesis. The depletion of HAUS1 in cells decreases the G2/M cell compartment and induces apoptosis. High HAUS1 expression was associated with several tumour-infiltrating immune cells such as Th2 cells, macrophages, and activated dendritic cells (PMID:35865089). 11094-2-AP can detect the 32 kDa band, which is consistent with the calculated MW.

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