Anticorps Polyclonal de lapin anti-HAX1

• Phare
• Validé par KD/KO

HAX1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 11266-1-AP

Synonymes

HAX 1, HAX1, HCLS1 associated protein X 1, HCLSBP1, HS1 associating protein X 1, HS1 binding protein 1, HS1BP1, HSP1BP 1, SCN3

---

Galerie de données de validation

• 

  WB analysis of HeLa using 11266-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11266-1-AP (HAX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11266-1-AP

  WB result of HAX1 antibody (11266-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAX1 transfected HEK-293 cells.

• 

  WB analysis of HeLa using 11266-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11266-1-AP (HAX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11266-1-AP

  IP Result of anti-HAX1 (IP:11266-1-AP, 3ug; Detection:11266-1-AP 1:300) with mouse brain tissue lysate 6000ug.

• 

  IHC staining of human lymphoma using 11266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11266-1-AP (HAX1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 11266-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11266-1-AP (HAX1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

11266-1-AP cible HAX1 dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, singe, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HAX1 Protéine recombinante Ag1792
• Nom complet: HCLS1 associated protein X-1
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC014314
• Symbole du gène: HAX1
• Identification du gène (NCBI): 10456
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HAX1, also named as HS1BP1, is a 32 kDa protein that is ubiquitously expressed. Hax1 has been demonstrated to be a negative regulator of apoptosis in many immune cell types. Furthermore, Hax1 has been shown to have additional roles in regulating cell motility and adhesion, and is overexpressed in many types of cancer. Kristina and Cavnar demonstrate in their paper validation of two research antibodies including Proteintech's anti-Hax1 (11266-1-AP). They have evidence that the rabbit anti-Hax1 (11266-1-AP, Proteintech Group Inc.) results in a more robust and consistent detection of Hax1.

Publications

Species	Application	Title
human	WB	Mol Cell; The IMiD target CRBN determines HSP90 activity toward transmembrane proteins essential in multiple myeloma.; Authors - Michael Heider
hamster	WB,IP	Nat Commun; Cerebellar Kv3.3 potassium channels activate TANK-binding kinase 1 to regulate trafficking of the cell survival protein Hax-1.; Authors - Yalan Zhang
human	WB	J Cell Biol; Hax1 regulates neutrophil adhesion and motility through RhoA.; Authors - Cavnar Peter J PJ ; KD Validated
mouse	WB	FASEB J; Suppression of Kv3.3 channels by antisense oligonucleotides reverses biochemical effects and motor impairment in spinocerebellar ataxia type 13 mice.; Authors - Yalan Zhang
human	WB, IHC	J Cell Mol Med; HAX1 maintains the glioma progression in hypoxia through promoting mitochondrial fission.; Authors - Jinghui Lin
human	WB	Fish Shellfish Immunol; Lamprey PHB2 maintains mitochondrial stability by tanslocation to the mitochondria under oxidative stress.; Authors - Ying Shi

Produits associés

Granzyme B Polyclonal antibody

13588-1-AP

RHOA Polyclonal antibody

10749-1-AP

GRB7 Polyclonal antibody

10045-1-Ig

XIAP Polyclonal antibody

10037-1-Ig

SERCA2 Polyclonal antibody

13985-1-AP

Cortactin Polyclonal antibody

11381-1-AP

ABCF1 Polyclonal antibody

13950-1-AP