Anticorps Polyclonal de lapin anti-HAX1

• Phare
• Validé par KD/KO

HAX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11266-1-PBS

Synonymes

HAX 1, HAX-1, HCLS1-associated protein X-1, HCLSBP1, HS1-associating protein X-1

---

Galerie de données de validation

• 

  WB analysis of HeLa using 11266-1-AP (same clone as 11266-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11266-1-AP (HAX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11266-1-AP (same clone as 11266-1-PBS)

  WB result of HAX1 antibody (11266-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAX1 transfected HEK-293 cells. This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11266-1-AP (same clone as 11266-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11266-1-AP (HAX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 11266-1-AP (same clone as 11266-1-PBS)

  IP result of anti-HAX1 (IP:11266-1-AP, 3ug; Detection:11266-1-AP 1:300) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 11266-1-AP (same clone as 11266-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11266-1-AP (HAX1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 11266-1-AP (same clone as 11266-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11266-1-AP (HAX1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11266-1-AP (same clone as 11266-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11266-1-AP (HAX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11266-1-AP (same clone as 11266-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HAX1 antibody (11266-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11266-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

11266-1-PBS cible HAX1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HAX1 Protéine recombinante Ag1792
• Nom complet: HCLS1 associated protein X-1
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC014314
• Symbole du gène: HAX1
• Identification du gène (NCBI): 10456
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HAX1, also named as HS1BP1, is a 32 kDa protein that is ubiquitously expressed. Hax1 has been demonstrated to be a negative regulator of apoptosis in many immune cell types. Furthermore, Hax1 has been shown to have additional roles in regulating cell motility and adhesion, and is overexpressed in many types of cancer. Kristina and Cavnar demonstrate in their paper validation of two research antibodies including Proteintech's anti-Hax1 (11266-1-AP). They have evidence that the rabbit anti-Hax1 (11266-1-AP, Proteintech Group Inc.) results in a more robust and consistent detection of Hax1.

{{ptg:RelatedPrimaryAntibodies}}