Anticorps Recombinant de lapin anti-Hemoglobin Alpha

Hemoglobin Alpha Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240049F12

N° de cat : 83185-5-RR

Synonymes

HBA1, Hemoglobin a, HBA2, Alpha-globin, Alpha globin

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Galerie de données de validation

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  WB analysis of human placenta using 83185-5-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 83185-5-RR (HBA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 83185-5-RR

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of TF-1 using 83185-5-RR

  1x10^6 TF-1 cells were intracellularly stained with 0.25 ug Hba1 Recombinant Antibody (83185-5-RR, Clone:240049F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83185-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83185-5-RR against Human HBA1 were performed. The affinity constant is 3.94 nM.

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Applications testées

• Résultats positifs en WB: tissu placentaire humain,
• Résultats positifs en FC (Intra): cellules K-562, cellules TF-1

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83185-5-RR cible Hemoglobin Alpha dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Hemoglobin Alpha Protéine recombinante Ag6040
• Nom complet: hemoglobin, alpha 1
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 14-16 kDa, 27 kDa
• Numéro d’acquisition GenBank: BC050661
• Symbole du gène: HBA1
• Identification du gène (NCBI): 3039
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.

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