Anticorps Polyclonal de lapin anti-HBP1

• Phare
• Validé par KD/KO

HBP1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 11746-1-AP

Synonymes

HBP1, HMG box containing protein 1, HMG box transcription factor 1

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Galerie de données de validation

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  WB analysis of human brain using 11746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11746-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11746-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11746-1-AP (HBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11746-1-AP

  IP Result of anti-HBP1 (IP:11746-1-AP, 4ug; Detection:11746-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules SGC-7901
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

11746-1-AP cible HBP1 dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HBP1 Protéine recombinante Ag2342
• Nom complet: HMG-box transcription factor 1
• Masse moléculaire calculée: 514 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC022329
• Symbole du gène: HBP1
• Identification du gène (NCBI): 26959
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HMG-box protein 1 (HBP1) is a transcriptional repressor, and has a role in cell cycle progression and tumor suppression. Also it's a repressor of the cyclin D1 gene and inhibits the Wnt signaling pathway. Interaction with RB1 enhances it's binding to the H1F0 promoter . Disrupts the interaction between DNA and TCF4. This is a rabbit polyclonal antibody raised against part of C-terminus of HBP1 of human origin.

Publications

Species	Application	Title
human	WB	Cancer Cell; MYC through miR-17-92 suppresses specific target genes to maintain survival, autonomous proliferation, and a neoplastic state.; Authors - Yulin Li
human,mouse	WB,IHC	Pharmacol Res; Targeting of HBP1/TIMP3 axis as a novel strategy against breast cancer; Authors - Yue Zhou
mouse,human	WB,IHC	Oncogene; miR-29c&b2 encourage extramedullary infiltration resulting in the poor prognosis of acute myeloid leukemia.; Authors - Yanyu Wei ; KD Validated
human	WB,IHC	Oncogene; CRISPR interference and activation of the microRNA-3662-HBP1 axis control progression of triple-negative breast cancer.; Authors - Baozhu Yi
human	WB	EMBO Rep; The human GID complex engages two independent modules for substrate recruitment.; Authors - Weaam I Mohamed
mouse	WB	J Cell Physiol; Transcription factor HMG box-containing protein 1 (HBP1) modulates mitotic clonal expansion (MCE) during adipocyte differentiation.; Authors - Chien-Yi Chan ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (11-22-2021)

This antibody gave a strong one band signal.

• Applications: Western Blot