Anticorps Recombinant de lapin anti-HBQ1

HBQ1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240527D6

N° de cat : 83576-4-PBS

Synonymes

240527D6, Hemoglobin subunit theta 1, Hemoglobin subunit theta-1, Hemoglobin theta 1 chain, Hemoglobin theta-1 chain

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Galerie de données de validation

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  WB analysis of K-562 using 83576-4-RR (same clone as 83576-4-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 83576-4-RR (HBQ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83576-4-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 83576-4-RR (same clone as 83576-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HBQ1 antibody (83576-4-RR, Clone: 240527D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83576-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83576-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 83576-4-PBS against Human HBQ1 was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 83576-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83576-4-RR against Human HBQ1 were performed. The affinity constant is 73.4 pM.

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Informations sur le produit

83576-4-PBS cible HBQ1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HBQ1 Protéine recombinante Ag10105
• Nom complet: hemoglobin, theta 1
• Masse moléculaire calculée: 142 aa, 16 kDa
• Poids moléculaire observé: 10~16 kDa
• Numéro d’acquisition GenBank: BC056686
• Symbole du gène: HBQ1
• Identification du gène (NCBI): 3049
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HBQ1 (Hemoglobin subunit theta-1) is a member of the human alpha-globin gene cluster. HBQ1 mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue (PMID: 3657976). HBQ1 gene was transcribed in an erythroleukemia cell line. HBQ1 has transcriptionally active and may be expressed in early erythroid tissue (PMID: 3422341).

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