Anticorps Polyclonal de lapin anti-HBS1L

• Phare
• Validé par KD/KO

HBS1L Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10359-1-PBS

Synonymes

EC:3.6.5.-, EF 1a, ERFS, HBS 1, HBS1

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Galerie de données de validation

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  WB analysis of Jurkat using 10359-1-AP (same clone as 10359-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10359-1-AP (same clone as 10359-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

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  WB analysis using 10359-1-AP (same clone as 10359-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10359-1-AP (same clone as 10359-1-PBS)

  WB result of HBS1L antibody (10359-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells. This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 10359-1-AP (same clone as 10359-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

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  WB analysis of A2780 using 10359-1-AP (same clone as 10359-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 10359-1-AP (HBS1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10359-1-AP (same clone as 10359-1-PBS)

  IP result of anti-HBS1L (IP:10359-1-AP, 4ug; Detection:10359-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 10359-1-AP (same clone as 10359-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10359-1-AP (HBS1L antibody) at dilution of 1:50 (under 25x lens). This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10359-1-AP (same clone as 10359-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HBS1L antibody (10359-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10359-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10359-1-PBS cible HBS1L dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HBS1L Protéine recombinante Ag0388
• Nom complet: HBS1-like (S. cerevisiae)
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC001465
• Symbole du gène: HBS1L
• Identification du gène (NCBI): 10767
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.

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