Anticorps Polyclonal de lapin anti-HBXIP

• Phare
• Validé par KD/KO

HBXIP Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 14492-1-AP

Synonymes

HBV X interacting protein, HBX interacting protein, HBXIP, LAMTOR5, XIP

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Galerie de données de validation

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  WB analysis using 14492-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14492-1-AP (HBXIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 14492-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 14492-1-AP (HBXIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 14492-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 14492-1-AP (HBXIP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 14492-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14492-1-AP (HBXIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14492-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14492-1-AP (HBXIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14492-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14492-1-AP (HBXIP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 14492-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14492-1-AP (HBXIP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 14492-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14492-1-AP (HBXIP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14492-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14492-1-AP (HBXIP antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of MCF-7 using 14492-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug HBXIP antibody (14492-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules U2OS
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu cardiaque humain, tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 8 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

14492-1-AP cible HBXIP dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HBXIP Protéine recombinante Ag5894
• Nom complet: hepatitis B virus x interacting protein
• Masse moléculaire calculée: 10 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC062619
• Symbole du gène: HBXIP
• Identification du gène (NCBI): 10542
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HBXIP, also named as XIP, belongs to the HBXIP family. It is originally identified by its interaction with the C-terminus of hepatitis B virus (HBV) X protein (HBx). HBXIP is a regulator of centrosome dynamics and cytokinesis. HBXIP is able to up-regulate S100A4 though activating STAT4 and inducing DNA methylation of PTEN.

Publications

Species	Application	Title
human	WB	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
human	WB	Front Oncol; Germacrone Regulates HBXIP-Mediated Cell Cycle, Apoptosis and Promotes the Formation of Autophagosomes to Inhibit the Proliferation of Gastric Cancer Cells.; Authors - Xing Fang
human	WB,IHC,IP	J Biol Chem; The oncoprotein HBXIP uses two pathways to up-regulate S100A4 in promotion of growth and migration of breast cancer cells.; Authors - Liu Shuangping S
human	WB,IHC	Cancer Lett; The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells.; Authors - Xu Fuqiang F ; KD Validated
human	WB,IHC	Int J Oncol; The oncoprotein hepatitis B X-interacting protein promotes the migration of ovarian cancer cells through the upregulation of S-phase kinase-associated protein 2 by Sp1.; Authors - Fuqiang Xu ; KD Validated
human	WB,IHC	Am J Cancer Res; Elevated HBXIP expression is associated with aggressive phenotype and poor prognosis in esophageal squamous cell carcinoma.; Authors - Honggang Xia

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