Anticorps Polyclonal de lapin anti-HCFC1

HCFC1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 19358-1-AP

Synonymes

C1 factor, CFF, HCF, HCF 1, HCF1, HCFC1, HFC1, Host cell factor 1, VCAF, VP16 accessory protein

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Galerie de données de validation

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  WB analysis of HEK-293 using 19358-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19358-1-AP (HCFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 19358-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19358-1-AP (HCFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19358-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19358-1-AP (HCFC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 19358-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19358-1-AP (HCFC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 19358-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19358-1-AP (HCFC1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

19358-1-AP cible HCFC1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HCFC1 Protéine recombinante Ag6798
• Nom complet: host cell factor C1 (VP16-accessory protein)
• Masse moléculaire calculée: 209 kDa
• Poids moléculaire observé: 230 kDa, 300 kDa
• Numéro d’acquisition GenBank: BC063435
• Symbole du gène: HCFC1
• Identification du gène (NCBI): 3054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Host cell factor 1 (HCFC1), also known as HCF-1, is a transcriptional co-regulator with a proteolytic cleavage at the PRO repeats that could be cleaved by O-GlcNAc transferase (OGT) to produce a heterodimeric complex of HCFC1N and HCFC1C subunits (PMID: 23539139). HCFC1 was first identified in herpes simplex virus transcription while it lacks a DNA binding domain. HCFC1 is not a traditional transcription factor, it interacts with multiple protein including transcription factors to regulate many biological processes, such as cell proliferation, migration, cell death and mitochondrial biogenesis (PMID: 25281006). Mutation in HCFC1 was related to an X-linked recessive disease ---cblX with a complex clinical manifestation involved in error of metabolism,  neurocognitive impairment and  craniofacial abnormalities (PMID: 24011988; PMID: 25281006).

Publications

Species	Application	Title
human,rat	IF	Microbiol Immunol; Susceptibility of rat immortalized neuronal cell line Rn33B expressing equine major histocompatibility class 1 to equine herpesvirus-1 infection is differentiation-dependent.; Authors - Erina Minato
mouse	WB	Adv Sci (Weinh); RONIN/HCF1-TFEB Axis Protects Against D-Galactose-Induced Cochlear Hair Cell Senescence Through Autophagy Activation; Authors - Yongjie Wei

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