Anticorps Polyclonal de lapin anti-HCFC1

HCFC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 28569-1-AP

Synonymes

HCF C-terminal chain 2, HCF C-terminal chain 1, HCF 1, HCF, CFF

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Galerie de données de validation

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  WB analysis using 28569-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28569-1-AP (HCFC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 28569-1-AP

  IP result of anti-HCFC1 (IP:28569-1-AP, 4ug; Detection:28569-1-AP 1:20000) with HeLa cells lysate 1600 ug.

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  IHC staining of mouse skin using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat cerebellum using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 28569-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28569-1-AP (HCFC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 28569-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HCFC1 antibody (28569-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C2C12, cellules C6, cellules K-562, cellules NIH/3T3, cellules PC-12
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu hépatique de souris, tissu cutané de rat, tissu cutané de souris, tissu de cancer du poumon humain, tissu de carcinome urothélial humain, tissu de cervelet de rat, tissu de cervelet de souris, tissu pulmonaire de rat, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28569-1-AP cible HCFC1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HCFC1 Protéine recombinante Ag29663
• Nom complet: host cell factor C1 (VP16-accessory protein)
• Masse moléculaire calculée: 209 kDa
• Poids moléculaire observé: 300 kDa, 100-150 kDa
• Numéro d’acquisition GenBank: NM_005334
• Symbole du gène: HCFC1
• Identification du gène (NCBI): 3054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HCFC1 (also known as HCF-1) was discovered in studies of herpes simplex virus (HSV) transcription. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in the control of the cell cycle and transcriptional regulation during herpes simplex virus infection. HCFC1 is a large protein of ∼300 kDa and is proteolytically processed into N- and C-terminal fragments of ∼150 kDa each.(PMID: 21295698 )

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