Anticorps Recombinant de lapin anti-HCLS1

HCLS1 Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242825E4

N° de cat : 85114-1-PBS

Synonymes

242825E4, CTTNL, Hematopoietic cell-specific LYN substrate 1, Hematopoietic lineage cell-specific protein, HS1

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Galerie de données de validation

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  WB analysis using 85114-1-RR (same clone as 85114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85114-1-RR (HCLS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85114-1-PBS in a different storage buffer formulation.

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  WB analysis of rat thymus using 85114-1-RR (same clone as 85114-1-PBS)

  rat thymus tissue were subjected to SDS PAGE followed by western blot with 85114-1-RR (HCLS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85114-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 85114-1-RR (same clone as 85114-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 85114-1-RR (HCLS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85114-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01860-1

  The mean HCLS1 concentration was determined to be 541.92 ng/mL in Ramos cell extract based on a 1.40 mg/mL extract load, 1,269.82 ng/mL in Daudi cell extract based on a 3.60 mg/mL extract load and 889.93 ng/mL in Jurkat cell extract based on a 3.10 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01860-1

  Sandwich ELISA standard curve of MP01860-1, Human HCLS1 Recombinant Matched Antibody Pair - PBS only. 85114-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag8645. 85114-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  IHC staining of mouse thymus using 85114-1-RR (same clone as 85114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 85114-1-RR (HCLS1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85114-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse thymus using 85114-1-RR (same clone as 85114-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 85114-1-RR (HCLS1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85114-1-PBS in a different storage buffer formulation.

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  IF Staining of HEL using 85114-1-RR (same clone as 85114-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEL cells using HCLS1 antibody (85114-1-RR, Clone: 242825E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85114-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 85114-1-RR (same clone as 85114-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HCLS1 antibody (85114-1-RR, Clone: 242825E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85114-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01860-1

  Cytometric bead array standard curve of MP01860-1, HCLS1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85114-1-PBS. Detection antibody: 85114-2-PBS. Standard: Ag8645. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 85114-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85114-1-RR against Human HCLS1 were performed. The affinity constant is 4.71 nM.

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Informations sur le produit

85114-1-PBS cible HCLS1 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HCLS1 Protéine recombinante Ag8645
• Nom complet: hematopoietic cell-specific Lyn substrate 1
• Masse moléculaire calculée: 486 aa, 54 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC016758
• Symbole du gène: HCLS1
• Identification du gène (NCBI): 3059
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Hematopoietic cell-specific Lyn substrate 1 (HS1, HCLS1, LckBP1 and p75) is a 75-kDa intracellular protein expressed in cells of lymphohemopoietic origin. It was originally identified in B-lymphocytes as a major substrate of B-cell receptor (BCR)-induced phosphorylation after antigen (Ag) stimulation. It's a substrate of the antigen receptor-coupled tyrosine kinase. HCLS1 plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells.

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