Anticorps Recombinant de lapin anti-HCN2

HCN2 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241601E10

N° de cat : 84308-3-PBS

Synonymes

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, HAC 1, Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2

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Galerie de données de validation

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  WB analysis using 84308-3-RR (same clone as 84308-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84308-3-RR (HCN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84308-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00160-2

  Sandwich ELISA standard curve of MP00160-2, Human HCN2 Recombinant Matched Antibody Pair - PBS only. 84308-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34038. 84308-3-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

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  Sample test of MP00160-2

  The mean HCN2 concentration was determined to be 9,765.2 ng/mL in rat brain tissue extract based on a 1.6 mg/mL extract load and 23,767.3 ng/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load.

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  Affinity and Kinetic Characterization of 84308-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84308-3-RR against Human HCN2 were performed. The affinity constant is 0.795 nM.

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Informations sur le produit

84308-3-PBS cible HCN2 dans les applications de WB, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HCN2 Protéine recombinante Ag34038
• Nom complet: hyperpolarization activated cyclic nucleotide-gated potassium channel 2
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: NM_001194
• Symbole du gène: HCN2
• Identification du gène (NCBI): 610
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HCN2, also named as BCNG2, belongs to the potassium channel HCN family. The molecular weight of immature HCN2 is 90 kDa, and the protein is about 120 kDa after n-terminal glycosylation. It has been found that 60 kDa of HCN2 protein can be identified in mouse heart samples. Although all protein isolation steps were handled on ice and in the presence of protease inhibitors to reduce protease-dependent effects and to minimize protein degradation, it is certainly possible that the presence of the 60 kDa HCN2 protein in the heart samples does, in fact, reflect protein degradation. (PMID:21796099, PMID: 19574228)

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