Anticorps Polyclonal de lapin anti-Orexin receptor 1

Orexin receptor 1 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 18370-1-AP

Synonymes

HCRTR1, hypocretin (orexin) receptor 1, Hypocretin receptor type 1, Orexin receptor 1, Orexin receptor type 1, Ox 1 R, Ox1 R, OX1R

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Galerie de données de validation

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  WB analysis of mouse brain using 18370-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18370-1-AP (Orexin receptor 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 18370-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 18370-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 18370-1-AP (Orexin receptor 1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 18370-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 18370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18370-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18370-1-AP (Orexin receptor 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 18370-1-AP

  1X10^6 SH-SY5Y cells were stained with 0.2ug Orexin receptor 1 antibody (18370-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules Jurkat, cellules SH-SY5Y, tissu pancréatique de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

18370-1-AP cible Orexin receptor 1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Orexin receptor 1 Protéine recombinante Ag12269
• Nom complet: hypocretin (orexin) receptor 1
• Masse moléculaire calculée: 425 aa, 48 kDa
• Poids moléculaire observé: 48-54 kDa
• Numéro d’acquisition GenBank: BC074796
• Symbole du gène: HCRTR1
• Identification du gène (NCBI): 3061
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The hypocretin receptor 1 (OX1R, also named HCRTR1) is a G protein-coupled receptor and is a critical participant in the regulation of motivated behavior (PMID:28971565). HCRTR1 is associated with the regulation of motivation, reward, and autonomic functions. HCRTR1 shows a selective binding affinity for HCRT1/orexin-A. Moreover, HCRTR1 and HCRTR2 share 64% sequence similarity in humans (PMID:34052813).

Publications

Species	Application	Title
mouse	WB,IHC	Front Pharmacol; The Ciji-Hua'ai-Baosheng II Formula Attenuates Chemotherapy-Induced Anorexia in Mice With H22 Hepatocellular Carcinoma.; Authors - Shengyan Xi
mouse	WB	Brain Res Bull; Aerobic training associated with an active lifestyle exerts a protective effect against oxidative damage in hypothalamus and liver: The involvement of energy metabolism.; Authors - Pedro P M Scariot
mouse	WB	Drug Des Devel Ther; Orexin-A Reverse Bone Mass Loss Induced by Chronic Intermittent Hypoxia Through OX1R-Nrf2/HIF-1α Pathway.; Authors - Hong Gu
rat	WB	Evid Based Complement Alternat Med; Soporific Effect of Modified Suanzaoren Decoction and Its Effects on the Expression of CCK-8 and Orexin-A.; Authors - Liang-Hui Zhan
human	WB	Biochim Biophys Acta Mol Basis Dis; Orexin-A/OX1R is involved in regulation of autophagy to promote cortisol secretion in adrenocortical cell; Authors - Xin Guo
mouse	IF	Nat Biotechnol; Generation of locus coeruleus norepinephrine neurons from human pluripotent stem cells; Authors - Yunlong Tao