Anticorps Monoclonal anti-HDAC1

HDAC1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4C4G7

N° de cat : 66085-1-PBS

Synonymes

4C4G7, EC:3.5.1.-, EC:3.5.1.98, GON 10, HD1

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Galerie de données de validation

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  WB analysis using 66085-1-Ig (same clone as 66085-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66085-1-Ig (HDAC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66085-1-Ig (same clone as 66085-1-PBS)

  Western blot analysis of HDAC1 in various cell lines using Proteintech antibody 66085-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66085-1-Ig (HDAC1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66085-1-Ig (HDAC1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66085-1-Ig (HDAC1 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDAC1 antibody (66085-1-Ig, Clone: 4C4G7 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDAC1 antibody (66085-1-Ig, Clone: 4C4G7 ) at dilution of 1:12000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66085-1-Ig (same clone as 66085-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66085-1-Ig(HDAC1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66085-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66085-1-PBS cible HDAC1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HDAC1 Protéine recombinante Ag19128
• Nom complet: histone deacetylase 1
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC000301
• Symbole du gène: HDAC1
• Identification du gène (NCBI): 3065
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calculated molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .

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