Anticorps Monoclonal anti-HDAC1

HDAC1 Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

Publications(1)

CloneNo.

4C4G7

N° de cat : CL647-66085

Synonymes

histone deacetylase 1, HD1, GON 10, EC:3.5.1.98, EC:3.5.1.-

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Galerie de données de validation

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  IF Staining of A431 using CL647-66085

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 647 HDAC1 antibody (CL647-66085, Clone: 4C4G7 ) at dilution of 1:1000.

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  IF Staining of A431 using CL647-66085

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 647 HDAC1 antibody (CL647-66085, Clone: 4C4G7 ) at dilution of 1:1000, CL488-Phalloidin (green).

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  FC experiment of HeLa using CL647-66085

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human HDAC1 (CL647-66085, Clone:4C4G7) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-66085 cible HDAC1 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HDAC1 Protéine recombinante Ag19128
• Nom complet: histone deacetylase 1
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC000301
• Symbole du gène: HDAC1
• Identification du gène (NCBI): 3065
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is raised against residues near the C terminus of human HDAC1. The calculated molecular weight of HDAC1 is 55 kDa, but modified HDAC1 is about 60-65 kDa .

Publications

Species	Application	Title
mouse		J Adv Res; Histone deacetylases facilitate Th17-cell differentiation and pathogenicity in autoimmune uveitis via CDK6/ID2 axis; Authors - Chun Zhang

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