Anticorps Polyclonal de lapin anti-HDAC1-specific

HDAC1-specific Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF,ELISA

Conjugaison

Non conjugué

N° de cat : 16160-1-AP

Synonymes

GON 10, HD1, HDAC1, histone deacetylase 1, RPD3, RPD3L1

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Galerie de données de validation

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  WB analysis of mouse testis using 16160-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 16160-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 16160-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16160-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16160-1-AP (HDAC1-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 16160-1-AP

  IP Result of anti-HDAC1-specific (IP:16160-1-AP, 4ug; Detection:16160-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IF Staining of HepG2 using 16160-1-AP

  Immunofluorescent analysis of HepG2 cells using 16160-1-AP (HDAC1-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu cérébral de souris, tissu de thymus de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16160-1-AP cible HDAC1-specific dans les applications de WB, IP, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone deacetylase 1
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_004964
• Symbole du gène: HDAC1
• Identification du gène (NCBI): 3065
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 1 was primarily found in the nucleus. This antibody is a rabbit polyclonal antibody raised against a peptide mapping near the N-terminus of human HDAC1 and is specific to HDAC1. It does not react with other HDACs.