Anticorps Monoclonal anti-HDAC10

HDAC10 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A1B11

N° de cat : 67646-1-PBS

Synonymes

1A1B11, EC:3.5.1.48, HD 10, HD10, HDAC 10

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Galerie de données de validation

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  WB analysis using 67646-1-Ig (same clone as 67646-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

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  WB analysis using 67646-1-Ig (same clone as 67646-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67646-1-Ig (HDAC10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 67646-1-Ig (same clone as 67646-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using HDAC10 antibody (67646-1-Ig, Clone: 1A1B11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67646-1-Ig (same clone as 67646-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using HDAC10 antibody (67646-1-Ig, Clone: 1A1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67646-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67646-1-PBS cible HDAC10 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HDAC10 Protéine recombinante Ag18470
• Nom complet: histone deacetylase 10
• Masse moléculaire calculée: 669 aa, 71 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC125083
• Symbole du gène: HDAC10
• Identification du gène (NCBI): 83933
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HDAC10, also named as Histone deacetylase 10, is a 669 amino acid protein, which is widely expressed with high levels in liver and kidney. HDAC10, which belongs to the class IIb subfamily, has an active deacetylase domain (DAC) and a catalytically inactive leucine-rich domain (LRD). HDAC10 is determined that it plays a role in homologous recombination, promotes autophagy and survival in neuroblastoma cells, suppresses cervical cancer metastasis and facilitates the cell cycle.

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