Anticorps Polyclonal de lapin anti-HDAC2

• Phare
• Validé par KD/KO

HDAC2 Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

N° de cat : CL555-12922

Synonymes

YAF1, RPD3, histone deacetylase 2, HD2, EC:3.5.1.98

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Galerie de données de validation

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  IF Staining of HepG2 using CL555-12922

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 HDAC2 antibody (CL555-12922) at dilution of 1:200.

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  FC experiment of HEK-293T using CL555-12922

  1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human HDAC2 (CL555-12922) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HEK-293T,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-12922 cible HDAC2 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HDAC2 Protéine recombinante Ag3607
• Nom complet: histone deacetylase 2
• Masse moléculaire calculée: 458 aa, 52 kDa; 488 aa,55 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC031055
• Symbole du gène: HDAC2
• Identification du gène (NCBI): 3066
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.

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