Anticorps Polyclonal de lapin anti-HDAC2-specific

HDAC2-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16152-1-AP

Synonymes

HD2, HDAC2, HDAC2-specific, histone deacetylase 2, RPD3, YAF1

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Galerie de données de validation

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  WB analysis of HepG2 using 16152-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 16152-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16152-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16152-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16152-1-AP (HDAC2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 16152-1-AP

  IP Result of anti-HDAC2-specific (IP:16152-1-AP, 4ug; Detection:16152-1-AP 1:500) with A431 cells lysate 2400ug.

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  IHC staining of human testis using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16152-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16152-1-AP

  Immunofluorescent analysis of HeLa cells using 16152-1-AP (HDAC2-specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules HeLa, tissu hépatique humain
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu testiculaire humain, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

16152-1-AP cible HDAC2-specific dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone deacetylase 2
• Masse moléculaire calculée: 458 aa, 52 kDa; 488 aa, 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: NM_001527
• Symbole du gène: HDAC2
• Identification du gène (NCBI): 3066
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC2 and is specific to HDAC2. It will not cross react with other HDACs.

Publications

Species	Application	Title
human		Hum Reprod; Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.; Authors - Yi-Min Cheng
human	WB	Chemosphere; Combined exposure of lead and cadmium leads to the aggravated neurotoxicity through regulating the expression of histone deacetylase 2.; Authors - Ruiqing Zhou
human	IF	Leuk Res; High-expressing cystic fibrosis transmembrane conductance regulator interacts with histone deacetylase 2 to promote the development of Ph+ leukemia through the HDAC2-mediated PTEN pathway.; Authors - Tianyou Yan
human	WB	Mol Cell; Histone H3 lysine 27 crotonylation mediates gene transcriptional repression in chromatin; Authors - Nan Liu