Anticorps Monoclonal anti-HDAC3

HDAC3 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

3E8F10

N° de cat : 67151-1-Ig

Synonymes

HD3, HDAC, HDAC3, histone deacetylase 3, RPD3, RPD3 2, SMAP45

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Galerie de données de validation

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  WB analysis using 67151-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67151-1-Ig (HDAC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67151-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67151-1-Ig (HDAC3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

67151-1-Ig cible HDAC3 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HDAC3 Protéine recombinante Ag28464
• Nom complet: histone deacetylase 3
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC000614
• Symbole du gène: HDAC3
• Identification du gène (NCBI): 8841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (-amino groups of lysines located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene.

Publications

Species	Application	Title
human	WB	Molecules; Anti-Colorectal Cancer Activity of Solasonin from Solanum nigrum L. via Histone Deacetylases-Mediated p53 Acetylation Pathway; Authors - Xintian Lan
human	WB,IP	J Biol Chem; The main protease of SARS-CoV-2 cleaves histone deacetylases and DCP1A, attenuating the immune defense of the interferon-stimulated genes; Authors - Liu Song
mouse	WB,IP	Exp Neurol; Neuronal HIPK2-HDAC3 axis regulates mitochondrial fragmentation to participate in stroke injury and post-stroke anxiety like behavior; Authors - Mengmeng Yang

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