Anticorps Recombinant de lapin anti-HDAC3

• Phare
• Validé par KD/KO

HDAC3 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2N1

N° de cat : 81211-1-PBS

Synonymes

2N1, EC:3.5.1.-, EC:3.5.1.98, HDAC 3, Histone deacetylase 3

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Galerie de données de validation

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  WB analysis using 81211-1-RR (same clone as 81211-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81211-1-RR (HDAC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

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  WB analysis of HAP1 using 81211-1-RR (same clone as 81211-1-PBS)

  WB result of HDAC3 antibody (81211-1-RR; 1:2000; room temperature for 1.5 hours) with wild-type and sg-HDAC3 transfected HAP1 cells This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 81211-1-RR (same clone as 81211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 81211-1-RR (same clone as 81211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 81211-1-RR (HDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81211-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81211-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81211-1-RR against Human HDAC3 were performed. The affinity constant is 0.213 nM.

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Informations sur le produit

81211-1-PBS cible HDAC3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HDAC3 Protéine recombinante Ag0396
• Nom complet: histone deacetylase 3
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC000614
• Symbole du gène: HDAC3
• Identification du gène (NCBI): 8841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes that regulate transcription by selectively deacetylating or acetylating the (-amino groups of lysines located near the amino termini of core histone proteins. At least 4 classes of HDAC were identified. HDAC3 is a class I HDAC. HDAC3 has histone deacetylase activity and may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. HDAC3 can also down-regulate p53 function and thus modulate cell growth and apoptosis. The gene encoding HDAC3 is regarded as a potential tumor suppressor gene.

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