Anticorps Polyclonal de lapin anti-HDAC3-specific

• Phare
• Validé par KD/KO

HDAC3-specific Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 16164-1-AP

Synonymes

HD3, HDAC3, HDAC3-specific, histone deacetylase 3, RPD3, RPD3 2, SMAP45

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Galerie de données de validation

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  WB analysis using 16164-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16164-1-AP (HDAC3-specific antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16164-1-AP

  WB result of HDAC3-specific antibody (16164-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HDAC3-specific transfected HeLa cells.

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  WB analysis of HEK-293 using 16164-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16164-1-AP (HDAC3-specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 16164-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 16164-1-AP (HDAC3-specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HEK-293, cellules HeLa, cellules HL-60, cellules MOLT-4, cellules NCI-H1299, tissu d'estomac humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16164-1-AP cible HDAC3-specific dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone deacetylase 3
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: NM_003883
• Symbole du gène: HDAC3
• Identification du gène (NCBI): 8841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

What is the molecular weight of HDAC3? Is HDAC3 post-translationally modified?

The molecular weight of HDAC3 is 49 kDa but HDAC3 can form homodimers and homotrimers in cells (PMID: 11779848). HDAC3 can be phosphorylated at S424 residue by protein kinase CK2, which regulates HDAC3 activity (PMID: 15805470).

 What is the subcellular localization of HDAC3?

Unlike other class I HDACs, HDAC3 actively shuttles between the nucleus and cytoplasm (PMID: 11779848). Additionally, HDAC3 can be present at the plasma membrane, where it associates with c-Src (PMID: 16532030).

 What is the tissue expression pattern of HDAC3?

Like other class I HDACs, HDAC3 is ubiquitously expressed (PMID: 9346952).

 What is the mechanism of HDAC3 in regulating gene expression?

Unlike other histone deacetylates, it exists in a complex with two proteins - nuclear receptor co-repressor (N-CoR) and silencing mediator for retinoid and thyroid receptors (SMRT) - and therefore acts as a mediator of the repression function of unliganded nuclear hormone receptors (PMID: 10809664). N-CoR and SMRT play an active role in the recruitment of HDAC3 to binding sites as well as directly regulating HDAC3 activity. Additionally, HDAC3 targets growth-regulated genes.

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