Anticorps Monoclonal anti-HDAC4

• Phare
• Validé par KD/KO

HDAC4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

1D2G12

Avis (1)

N° de cat : 66838-1-Ig

Synonymes

KIAA0288, HD4, EC:3.5.1.98, 1D2G12

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Galerie de données de validation

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  WB analysis using 66838-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66838-1-Ig (HDAC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66838-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66838-1-Ig (HDAC4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66838-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66838-1-Ig (HDAC4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 66838-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using HDAC4 antibody (66838-1-Ig, Clone: 1D2G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of C2C12 using 66838-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using HDAC4 antibody (66838-1-Ig, Clone: 1D2G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de souris,
• Résultats positifs en IF/ICC: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

66838-1-Ig cible HDAC4 dans les applications de WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HDAC4 Protéine recombinante Ag11345
• Nom complet: histone deacetylase 4
• Masse moléculaire calculée: 972 aa, 106 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC039904
• Symbole du gène: HDAC4
• Identification du gène (NCBI): 9759
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

Publications

Species	Application	Title
human	WB	Oncogenesis; USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.; Authors - Wei Zhan
human	IF	Virulence; Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.; Authors - Wei Jie
human	IF	J Mol Cell Cardiol; Cellular heterogeneity and immune microenvironment revealed by single-cell transcriptome in venous malformation and cavernous venous malformation.; Authors - Yongyun Li
mouse	WB,IP,ChIP	Appl Physiol Nutr Metab; Exercise improves high-fat diet-induced metabolic disorder by promoting HDAC5 degradation through the ubiquitin-proteasome system in skeletal muscle; Authors - Song Huang ; KD Validated
mouse	WB	J Toxicol Sci; Propofol improves ischemia reperfusion-induced liver fibrosis by regulating lncRNA HOXA11-AS; Authors - Jia Luo ; KD Validated
human,mouse	WB	Adv Clin Exp Med; Tasquinimod enhances the sensitivity of ovarian cancer cells to cisplatin by regulating the Nur77-Bcl-2 apoptotic pathway; Authors - Ying Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford (Verified Customer) (10-26-2023)

In our screening of basic expression in lung, breast, and gastric cancer cell lines, antibody concentrations ranging from 1:1000 to 2000 all displayed clear bands, indicating stable working conditions.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Multiple cell lines of lung cancer, breast cancer, gastric cancer.

HDAC4 Antibody Western Blot validation (1:1000 dilution) in Multiple cell lines of lung cancer, breast cancer, gastric cancer. (Cat no:66838-1-Ig)

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