Anticorps Monoclonal anti-HDAC4

• Phare
• Validé par KD/KO

HDAC4 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D2G12

N° de cat : 66838-1-PBS

Synonymes

KIAA0288, HD4, EC:3.5.1.98, 1D2G12

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Galerie de données de validation

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  WB analysis using 66838-1-Ig (same clone as 66838-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66838-1-Ig (HDAC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66838-1-Ig (same clone as 66838-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66838-1-Ig (HDAC4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66838-1-Ig (same clone as 66838-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66838-1-Ig (HDAC4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 66838-1-Ig (same clone as 66838-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using HDAC4 antibody (66838-1-Ig, Clone: 1D2G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 66838-1-Ig (same clone as 66838-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using HDAC4 antibody (66838-1-Ig, Clone: 1D2G12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66838-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66838-1-PBS cible HDAC4 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HDAC4 Protéine recombinante Ag11345
• Nom complet: histone deacetylase 4
• Masse moléculaire calculée: 972 aa, 106 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC039904
• Symbole du gène: HDAC4
• Identification du gène (NCBI): 9759
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.

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