Anticorps Polyclonal de lapin anti-HDAC4-specific

• Phare
• Validé par KD/KO

HDAC4-specific Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 16165-1-AP

Synonymes

HDAC4, KIAA0288, HD4, EC:3.5.1.98

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Galerie de données de validation

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  WB analysis using 16165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16165-1-AP (HDAC4-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16165-1-AP

  IP result of anti-HDAC4-specific (IP:16165-1-AP, 4ug; Detection:16165-1-AP 1:500) with HeLa cells lysate 2440ug.

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  IHC staining of human ovary cancer using 16165-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16165-1-AP (HDAC4-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16165-1-AP (HDAC4-specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary cancer using 16165-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 16165-1-AP (HDAC4-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16165-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16165-1-AP (HDAC4-specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of mouse liver using 16165-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using HDAC4-specific antibody (16165-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: human ovary cancer tissue, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

16165-1-AP cible HDAC4-specific dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone deacetylase 4
• Masse moléculaire calculée: 119 kDa
• Poids moléculaire observé: 119-140 kDa
• Numéro d’acquisition GenBank: BC039904
• Symbole du gène: HDAC4
• Identification du gène (NCBI): 9759
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HDAC4, also named as HDACA, belongs to the histone deacetylase family. HDAC4 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC4 is Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human HDAC4.

Publications

Species	Application	Title
human,mouse	IF	Nat Cell Biol; Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.; Authors - Yoshihiko Kobayashi
human	WB	Hepatology; The histone deacetylase 4/SP1/microrna-200a regulatory network contributes to aberrant histone acetylation in hepatocellular carcinoma.; Authors - Yuan Ji-hang JH ; KD Validated
mouse	WB	J Orthop Translat; Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta; Authors - Bin Sun
mouse	WB	Biol Trace Elem Res; Exercise Alleviates Fluoride-Induced Learning and Memory Impairment in Mice: Role of miR-206-3p and PREG; Authors - Lei Chai
		Am J Physiol Lung Cell Mol Physiol; Complex urban atmosphere alters alveolar stem cells niche properties and drives lung fibrosis; Authors - Randa Belgacemi

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