Anticorps Monoclonal anti-HDAC5

HDAC5 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B9E6

N° de cat : 68437-1-PBS

Synonymes

Antigen NY CO 9, FLJ90614, HD5, HDAC5, histone deacetylase 5, KIAA0600, NY CO 9

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Galerie de données de validation

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  WB analysis using 68437-1-Ig (same clone as 68437-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68437-1-Ig (HDAC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68437-1-PBS in a different storage buffer formulation.

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  WB analysis using 68437-1-Ig (same clone as 68437-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 68437-1-Ig (HDAC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68437-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68437-1-PBS cible HDAC5 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HDAC5 Protéine recombinante Ag30557
• Nom complet: histone deacetylase 5
• Masse moléculaire calculée: 122 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: NM_005474
• Symbole du gène: HDAC5
• Identification du gène (NCBI): 10014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.

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