Anticorps Polyclonal de lapin anti-HDAC5-specific

• Phare
• Validé par KD/KO

HDAC5-specific Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 16166-1-AP

Synonymes

Antigen NY CO 9, HD5, HDAC5, histone deacetylase 5, KIAA0600, NY CO 9

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Galerie de données de validation

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  WB analysis of HeLa using 16166-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16166-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 16166-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 16166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 16166-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 16166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 16166-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16166-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16166-1-AP (HDAC5-specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16166-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16166-1-AP (HDAC5-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:100-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• CoIP: See 3 publications below

Informations sur le produit

16166-1-AP cible HDAC5-specific dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone deacetylase 5
• Masse moléculaire calculée: 122 kDa
• Poids moléculaire observé: 120-140 kDa
• Numéro d’acquisition GenBank: BC051824
• Symbole du gène: HDAC5
• Identification du gène (NCBI): 10014
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. This antibody only binds HDAC5. It does not cross-react with other HDACs.

Publications

Species	Application	Title
human	WB	Cell; Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.; Authors - Xun Huang
mouse, rat	WB,IHC,IF	Kidney Int; Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.; Authors - Xiaojie Wang
human	WB	Cancer Lett; ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer; Authors - Chen Zhou
human	WB	J Cell Mol Med; Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.; Authors - Shoujie Feng ; KD Validated
rat	WB,IHC	J Cell Mol Med; HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury.; Authors - He Min M
rat	WB,IF	Biomed Pharmacother; Trichostatin A increases BDNF protein expression by improving XBP-1s/ATF6/GRP78 axis in Schwann cells of diabetic peripheral neuropathy.; Authors - Jiahui An ; KD Validated