Anticorps Polyclonal de lapin anti-HDAC6-specific

HDAC6-specific Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16167-1-AP

Synonymes

HD6, HDAC6, histone deacetylase 6, JM21, KIAA0901

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Galerie de données de validation

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  WB analysis of MCF-7 using 16167-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16167-1-AP (HDAC6-specific antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16167-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16167-1-AP (HDAC6-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16167-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16167-1-AP (HDAC6-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16167-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16167-1-AP (HDAC6-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 16167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16167-1-AP (HDAC6-specific antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 16167-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16167-1-AP (HDAC6-specific antibody at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules MCF7, cellules HT-1080, cellules MCF-7
• Résultats positifs en IHC: tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

16167-1-AP cible HDAC6-specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: histone deacetylase 6
• Masse moléculaire calculée: 131 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC005872
• Symbole du gène: HDAC6
• Identification du gène (NCBI): 10013
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. HDAC6 is a member of the class II mammalian histone deacetylases. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. This antibody is specific to HDAC6. It does not cross react with other HDACs.

Publications

Species	Application	Title
mouse	WB,IHC	Oncogene; MicroRNA-22 represses glioma development via activation of macrophage-mediated innate and adaptive immune responses.; Authors - Jiajie Tu
rat	WB	J Cell Mol Med; HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury.; Authors - He Min M