Anticorps Polyclonal de lapin anti-HDDC3

HDDC3 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 21091-1-AP

Synonymes

HD domain containing 3, HD domain containing protein 3, HDDC3, MESH1

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Galerie de données de validation

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  WB analysis using 21091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 21091-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21091-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21091-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21091-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 21091-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 21091-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21091-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP (HDDC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21091-1-AP

  IP result of anti-HDDC3 (IP:21091-1-AP, 4ug; Detection:21091-1-AP 1:500) with HeLa cells lysate 3400ug.

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  IHC staining of human prostate cancer using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21091-1-AP (HDDC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 21091-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21091-1-AP (HDDC3 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules HeLa, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain, tissu pancréatique de souris, tissu pulmonaire de souris, tissu rénal de souris, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cérébral humain, tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

21091-1-AP cible HDDC3 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HDDC3 Protéine recombinante Ag15342
• Nom complet: HD domain containing 3
• Masse moléculaire calculée: 179 aa, 20 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC033794
• Symbole du gène: HDDC3
• Identification du gène (NCBI): 374659
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MESH1, encodes by HDDC3 in human and Q9VAM9 in Drosophila melanogaster, was identified as functional SpoT orthologs in metazoan (PMID: 20818390). MESH1 is a cytosolic NADPH phosphatase that is induced under stress conditions, leading to the NADPH depletion and ferroptosis-a novel form of iron-dependent regulated cell death characterized by lipid peroxidation. Accordingly, MESH1 removal preserves the NADPH level in stressed cells and promotes their ferroptotic survival(PMID: 32462112, PMID: 34294679).

Publications

Species	Application	Title
human	WB	Cell Death Dis; MESH1 knockdown triggers proliferation arrest through TAZ repression.; Authors - Tianai Sun
mouse	WB	Biomolecules; Molecular Profiling of Mouse Models of Loss or Gain of Function of the KCNT1 (Slack) Potassium Channel and Antisense Oligonucleotide Treatment; Authors - Fangxu Sun

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