Anticorps Monoclonal anti-HDGF

HDGF Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5A7G3

N° de cat : 60064-1-PBS

Synonymes

5A7G3, Hepatoma-derived growth factor, High mobility group protein 1-like 2, HMG-1L2

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Galerie de données de validation

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  WB analysis of SMMC-7721 using 60064-1-Ig (same clone as 60064-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 60064-1-Ig (same clone as 60064-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60064-1-Ig (same clone as 60064-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 60064-1-Ig (same clone as 60064-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60064-1-Ig (same clone as 60064-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 60064-1-Ig (same clone as 60064-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 60064-1-Ig (HDGF Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60064-1-Ig (same clone as 60064-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60064-1-Ig (HDGF Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60064-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60064-1-PBS cible HDGF dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HDGF Protéine recombinante Ag1893
• Nom complet: hepatoma-derived growth factor (high-mobility group protein 1-like)
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC018991
• Symbole du gène: HDGF
• Identification du gène (NCBI): 3068
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HDGF, also named as HMG1L2, belongs to the HDGF family. It is a heparin-binding protein, with mitogenic activity for fibroblasts. HDGF acts as a transcriptional repressor. It plays important roles in the development and progression of cancers. HDGF may have a synergistic effect with VEGF during cancer development and Progression. (PMID:20848225)

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