Anticorps Polyclonal de lapin anti-HDGF2

• Phare
• Validé par KD/KO

HDGF2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

IHC, IF/ICC, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications(15)

Avis (1)

N° de cat : 15134-1-AP

Synonymes

HDGFRP2, HRP 2, Hepatoma-derived growth factor 2, HDGF-related protein 2, HDGFL2

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Galerie de données de validation

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  IHC staining of human colon using 15134-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15134-1-AP (HRP 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 15134-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15134-1-AP (HRP 2 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 15134-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using HDGF2 antibody (15134-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 15134-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HDGF2 antibody (15134-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 15134-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15134-1-AP (HRP 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules A431

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

15134-1-AP cible HDGF2 dans les applications de IHC, IF/ICC, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HDGF2 Protéine recombinante Ag7281
• Nom complet: hepatoma-derived growth factor-related protein 2
• Masse moléculaire calculée: 74 kDa
• Numéro d’acquisition GenBank: BC000755
• Symbole du gène: HDGF2
• Identification du gène (NCBI): 84717
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HDGF2, also known as HDGFL2 (Hepatoma-Derived Growth Factor-like 2), is a gene that encodes a member of the hepatoma-derived growth factor (HDGF) family. HDGF2 plays a role in LEDGF/p75-independent HIV-1 replication by determining HIV-1 integration site selection. It is also involved in the binding to methylated histones, which suggests a role in epigenetic regulation.

Publications

Species	Application	Title
human	WB,IF	Sci Transl Med; Mis-spliced transcripts generate de novo proteins in TDP-43-related ALS/FTD; Authors - Sahba Seddighi
human	WB	Sci Adv; The H3K36me2 writer-reader dependency in H3K27M-DIPG.; Authors - Jia-Ray Yu ; KD Validated
human	WB	Sci Adv; LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells.; Authors - Gary LeRoy
mouse	WB,chIP	Nucleic Acids Res; HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.; Authors - Xu Zhu ; KD Validated
human	IF, IP	Nucleic Acids Res; Hepatoma-derived growth factor-related protein 2 promotes DNA repair by homologous recombination.; Authors - Annika Baude
human		Mov Disord; TDP-43 Cryptic RNAs in Perry Syndrome: Differences across Brain Regions and TDP-43 Proteinopathies; Authors - Sarah R Pickles

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (12-04-2023)

Works in ICC (nuclear staining) and WB (no non specific bands)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human / SH-SY5Y cells

HDGF2 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Human / SH-SY5Y cells (Cat no:15134-1-AP)

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