Anticorps Monoclonal anti-HE4

HE4 Monoclonal Antibody for IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5B2D9

N° de cat : 66557-1-PBS

Synonymes

WFDC2, 5B2D9, Epididymal secretory protein E4, Major epididymis-specific protein E4, WAP four disulfide core domain protein 2

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Galerie de données de validation

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  IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66557-1-Ig (same clone as 66557-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

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  IF Staining of SKOV-3 using 66557-1-Ig (same clone as 66557-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using HE4 antibody (66557-1-Ig, Clone: 5B2D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66557-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51083-2

  Cytometric bead array standard curve of MP51083-2, HE4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66557-1-PBS. Detection antibody: 66557-3-PBS. Standard:Ag5936. Range: 0.781-100 ng/mL.

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Informations sur le produit

66557-1-PBS cible HE4 dans les applications de IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HE4 Protéine recombinante Ag5936
• Nom complet: WAP four-disulfide core domain 2
• Masse moléculaire calculée: 13 kDa
• Numéro d’acquisition GenBank: BC046106
• Symbole du gène: HE4
• Identification du gène (NCBI): 10406
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.

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