Anticorps Recombinant de lapin anti-HEBP1

• Phare
• Validé par KD/KO

HEBP1 Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230156B6

N° de cat : 82950-1-PBS

Synonymes

Heme-binding protein 1, heme binding protein 1, HEBP 1, HEBP, HBP

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Galerie de données de validation

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  WB analysis using 82950-1-RR (same clone as 82950-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82950-1-RR (HEBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82950-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 82950-1-RR (same clone as 82950-1-PBS)

  WB result of HEBP1 antibody (82950-1-RR; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEBP1 transfected A549 cells. This data was developed using the same antibody clone with 82950-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 82950-1-RR (same clone as 82950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82950-1-RR (HEBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82950-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 82950-1-RR (same clone as 82950-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82950-1-RR (HEBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82950-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82950-1-RR (same clone as 82950-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug HEBP1 Recombinant antibody (82950-1-RR, Clone:230156B6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82950-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82950-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82950-1-RR against Human HEBP1 were performed. The affinity constant is 25.2 nM.

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Informations sur le produit

82950-1-PBS cible HEBP1 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: HEBP1 Protéine recombinante Ag10538
• Nom complet: heme binding protein 1
• Masse moléculaire calculée: 189 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC016277
• Symbole du gène: HEBP1
• Identification du gène (NCBI): 50865
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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