Anticorps Polyclonal de lapin anti-HECTD1

• Phare
• Validé par KD/KO

HECTD1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20605-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 20605-1-AP (same clone as 20605-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20605-1-AP (HECTD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 20605-1-AP (same clone as 20605-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 20605-1-AP (HECTD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 20605-1-AP (same clone as 20605-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 20605-1-AP (HECTD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20605-1-AP (same clone as 20605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20605-1-AP (HECTD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20605-1-AP (same clone as 20605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20605-1-AP (HECTD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20605-1-AP (same clone as 20605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 20605-1-AP (same clone as 20605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20605-1-AP (same clone as 20605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20605-1-AP (same clone as 20605-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20605-1-AP (HECTD1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20605-1-AP (same clone as 20605-1-PBS)

  Immunofluorescent analysis of Hela cells, using HECTD1 antibody 20605-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20605-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

20605-1-PBS cible HECTD1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HECTD1 Protéine recombinante Ag14581
• Nom complet: HECT domain containing 1
• Masse moléculaire calculée: 2612 aa, 290 kDa
• Poids moléculaire observé: 290 kDa
• Numéro d’acquisition GenBank: BC006237
• Symbole du gène: HECTD1
• Identification du gène (NCBI): 25831
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

HECTD1 (E3 ubiquitin-protein ligase), which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates,may be required for development of the head mesenchyme and neural tube closure.It is also involved in sporadic Parkinson's disease(PMID:16714300).This protein is specific to HECTD1.

{{ptg:RelatedPrimaryAntibodies}}