Anticorps Polyclonal de lapin anti-HECTD3

• Phare
• Validé par KD/KO

HECTD3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 11487-1-AP

Synonymes

RP11 69J16.1, HECT-type E3 ubiquitin transferase HECTD3, HECT domain-containing protein 3, HECT domain containing 3, EC:2.3.2.26

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Galerie de données de validation

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  WB analysis using 11487-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11487-1-AP (HECTD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11487-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11487-1-AP (HECTD3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of MDA-MB-453s using 11487-1-AP

  IP result of anti-HECTD3 (IP:11487-1-AP, 4ug; Detection:11487-1-AP 1:300) with MDA-MB-453s cells lysate 2800ug.

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  IHC staining of human stomach using 11487-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11487-1-AP (HECTD3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 11487-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11487-1-AP (HECTD3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules MDA-MB-453s
• Résultats positifs en IP: cellules MDA-MB-453s
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11487-1-AP cible HECTD3 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HECTD3 Protéine recombinante Ag1998
• Nom complet: HECT domain containing 3
• Masse moléculaire calculée: 861 aa, 97 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC019105
• Symbole du gène: HECTD3
• Identification du gène (NCBI): 79654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HECTD3(HECT domain-containing protein 3) may be involved in the regulation of mitotic metaphase/anaphase transition. It can specifically ubiquitinate TRIOBP and target it for proteasomal degradation and it can also mediates the ubiquitination of STX8. HECTD3 is a new oncogenic E3 ligase in human prostate cancer.( Li Y, etc. 2008) HECTD3 may facilitate cell cycle progression via regulating ubiquitination and degradation of Tara(PMID:18194665). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse,human	WB	Nat Commun; E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3.; Authors - Peng Gao
human	WB	Front Oncol; uc.77- Downregulation Promotes Colorectal Cancer Cell Proliferation by Inhibiting FBXW8-Mediated CDK4 Protein Degradation.; Authors - Zhijian Zheng
human	WB	Cell Death Discov; HECTD3 promotes gastric cancer progression by mediating the polyubiquitination of c-MYC.; Authors - Guanghui Zhang ; KD Validated
human,mouse	WB,IP	Br J Cancer; HECTD3 regulates the tumourigenesis of glioblastoma by polyubiquitinating PARP1 and activating EGFR signalling pathway; Authors - Guanghui Zhang ; KD Validated
human	CoIP	Cell Death Dis; Radiation induces IRAK1 expression to promote radioresistance by suppressing autophagic cell death via decreasing the ubiquitination of PRDX1 in glioma cells; Authors - Jing Li
mouse	IF	Cell Death Dis; HECTD3 inhibits NLRP3 inflammasome assembly and activation by blocking NLRP3-NEK7 interaction; Authors - Zhuo Cheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wei (Verified Customer) (01-23-2020)

Good, single band

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12

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