Anticorps Polyclonal de lapin anti-HECW2

HECW2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 32026-1-AP

Synonymes

NEDD4-like E3 ubiquitin-protein ligase 2, KIAA1301, HECT-type E3 ubiquitin transferase HECW2, HECT, C2 and WW domain-containing protein 2, EC:2.3.2.26

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Galerie de données de validation

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  WB analysis using 32026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32026-1-AP (HECW2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 32026-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32026-1-AP (HECW2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat cerebellum using 32026-1-AP

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 32026-1-AP (HECW2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 32026-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HECW2 antibody (32026-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-468, U-118 MG cells
• Résultats positifs en IHC: tissu de cervelet de souris, tissu de cervelet de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32026-1-AP cible HECW2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HECW2 Protéine recombinante Ag36316
• Nom complet: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
• Masse moléculaire calculée: 176 kDa，1572aa
• Poids moléculaire observé: 240 kDa
• Numéro d’acquisition GenBank: XM_017004563.1
• Symbole du gène: HECW2
• Identification du gène (NCBI): 57520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HECW2, also known as NEDL2 (Nedd4-like E3 ubiquitin-protein ligase 2), is a member of the HECT (Homologous to the E6-AP Carboxyl Terminus) family of E3 ubiquitin ligases. It is crucial in various pathophysiological processes, including cell proliferation, apoptosis, autophagy, immune response, and DNA damage repair (PMID: 39529070). HECW2 is known to stabilize and enhance the transcriptional activity of p73, a member of the p53 family of tumor suppressors, which promotes cell cycle arrest and apoptosis and has an essential role in neurodevelopment (PMID: 27389779). In cancer, HECW2 is a promising target for tumor therapy due to its role in regulating cell cycle progression and angiogenesis (PMID: 39529070). In neurodevelopmental disorders, variants in HECW2 have been reported to cause a disorder characterized by hypotonia, seizures, and impaired language (PMID: 34321324).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nikolett (Verified Customer) (09-25-2025)

This antibody is working on human lung cancer cell lines for Western Blotting after overnight incubation. The band was a bit faint but after exposure improvements I achieved a nice image. I recommend this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Lung Cancer Cell Lines

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