Anticorps Monoclonal anti-HELIOS

HELIOS Monoclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F1F10

N° de cat : 68558-1-PBS

Synonymes

HELIOS, IKZF2, Zinc finger protein Helios, ZNF1A2, ZNFN1A2

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Galerie de données de validation

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  WB analysis of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (HELIOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

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  WB analysis of MOLT-4 using 68558-1-Ig (same clone as 68558-1-PBS)

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 68558-1-Ig (IKZF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 68558-1-Ig (same clone as 68558-1-PBS)

  IP result of anti-HELIOS (IP:68558-1-Ig, 4ug; Detection:68558-1-Ig 1:3000) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 68558-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68558-1-PBS cible HELIOS dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: HELIOS Protéine recombinante Ag4357
• Nom complet: IKAROS family zinc finger 2 (Helios)
• Masse moléculaire calculée: 500 aa, 57 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC028936
• Symbole du gène: HELIOS
• Identification du gène (NCBI): 22807
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IKZF2 (Helios), a member of the Ikaros transcription factor family, is expressed in a majority of Treg cells and some T cell and lymphocyte subsets. The expression of IKZF2 is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685). IKZF2 plays a crucial role in maintaining function and stability of Treg cells, and IKZF2 deficiency reduces tumor growth in mice (PMID: 36863346).

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