Anticorps Polyclonal de lapin anti-HELLS

HELLS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (2)

N° de cat : 11955-1-AP

Synonymes

EC:3.6.4.-, helicase, lymphoid, LSH, Lymphoid specific helicase, Lymphoid-specific helicase

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Galerie de données de validation

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  WB analysis of K-562 using 11955-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11955-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11955-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11955-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 11955-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 11955-1-AP (HELLS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 11955-1-AP

  IP result of anti-HELLS (IP:11955-1-AP, 4ug; Detection:11955-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of mouse testis using 11955-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11955-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 11955-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 11955-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 11955-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 11955-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 11955-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 11955-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 11955-1-AP (HELLS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11955-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HELLS antibody (11955-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 11955-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HELLS Polyclonal antibody (11955-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules DU 145, cellules PC-3, tissu de thymus de souris, tissu testiculaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de thymus de souris, tissu de thymus de rat, tissu testiculaire de rat, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IF: See 4 publications below

Informations sur le produit

11955-1-AP cible HELLS dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HELLS Protéine recombinante Ag2558
• Nom complet: helicase, lymphoid-specific
• Masse moléculaire calculée: 348 aa, 40 kDa, 97 kDa
• Poids moléculaire observé: 97 kDa
• Numéro d’acquisition GenBank: BC031004
• Symbole du gène: HELLS
• Identification du gène (NCBI): 3070
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lymphoid-specific helicase(HELLS) is a member of the SNF2 family of chromatin remodeling proteins that is required for development and survival. It's required for de novo or maintenance DNA methylation, which mediated the silencing of the imprinted CDKN1C gene. Also it involved in formation and organization of heterochromatin that regulate transcription and mitosis.

Publications

Species	Application	Title
	WB	Nat Commun; Germinal center output is sustained by HELLS-dependent DNA-methylation-maintenance in B cells; Authors - Clara Cousu
mouse	WB,IF	Nucleic Acids Res; The C-terminal 4CXXC-type zinc finger domain of CDCA7 recognizes hemimethylated DNA and modulates activities of chromatin remodeling enzyme HELLS; Authors - Akeo Shinkai
mouse	WB	Sci Adv; CDCA7-associated global aberrant DNA hypomethylation translates to localized, tissue-specific transcriptional responses; Authors - Maja Vukic
mouse	WB,IF	Genome Biol; Lsh regulates LTR retrotransposon repression independently of Dnmt3b function.; Authors - Donncha S Dunican
human	WB	mSystems; Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes; Authors - Joseph M. Dybas
		Hum Mol Genet; Converging disease genes in ICF syndrome: ZBTB24 controls expression of CDCA7 in mammals.; Authors - Haoyu Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Diana (Verified Customer) (08-18-2022)

In IF the antibody binds specific in the nucleus and has low background, thats great! In WB I have a band as aspected around 100kd but I also have some other smaller unspecific bands. Here I will work on the troubleshooting. Thanks for the corresponding protocolls with buffers and incubation time.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500 (WB), 1:25 (IF)
• Cell Tissue Type: mouse testis, GC1, NIH3T3

FH

Michelle (Verified Customer) (10-24-2019)

Great antibody for HELLS. Customer support was really helpful when I was troubleshooting how to clean up my western blot. Changed the path of my project since multiple bands for different isoforms of the protein appeared in the cell lines I was invesitgating.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, Jurkats, HAP1, PBMC

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