Anticorps Polyclonal de lapin anti-HELZ

HELZ Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

Avis (2)

N° de cat : 26635-1-AP

Synonymes

DHRC, DRHC, helicase with zinc finger, HELZ, HUMORF5, KIAA0054

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Galerie de données de validation

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  WB analysis of HeLa using 26635-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26635-1-AP (HELZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26635-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26635-1-AP (HELZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 26635-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26635-1-AP (HELZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26635-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26635-1-AP (HELZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 26635-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 26635-1-AP (HELZ antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

26635-1-AP cible HELZ dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HELZ Protéine recombinante Ag24326
• Nom complet: helicase with zinc finger
• Masse moléculaire calculée: 1942 aa, 219 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC130582
• Symbole du gène: HELZ
• Identification du gène (NCBI): 9931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ramona (Verified Customer) (11-12-2019)

It worked well for WB as I know which band to look at based on molecular weight. It stains a lot of non-specific molecules in IF, which seem to include something like actin fibers

• Applications: Immunofluorescence,
• Primary Antibody Dilution: IF 1:1000, WB 1:600
• Cell Tissue Type: Human U2OS cell line

HELZ Antibody Immunofluorescence, validation (IF 1:1000, WB 1:600 dilution) in Human U2OS cell line (Cat no:26635-1-AP)

FH

Ramona (Verified Customer) (09-04-2019)

Our own custom antibody: I used an antibody customary made by a commercial company. The best crude serum of our choice was affinity purified.Problems: los of non-specific bands, cannot re-use antibody solution for the next membrane. Sometimes does not work and do not show any band even in control.Proteintech antibody: strong band of specific protein. Knock down shows expression but reduced compared to controls, which is more realistic than zero expression in all the cells.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1/600
• Cell Tissue Type: U2OS cell

HELZ Antibody Western Blot,Immunofluorescence, validation (1/600 dilution) in U2OS cell (Cat no:26635-1-AP)