Anticorps Recombinant de lapin anti-HER2/ErbB2

HER2/ErbB2 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242436C4

N° de cat : 98304-3-PBS

Synonymes

ERBB2, CD340, HER 2, HER 2/neu, HER2

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Galerie de données de validation

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  FC experiment of MCF-7 using 98304-3-RR (same clone as 98304-3-PBS)

  1x10^6 MCF-7 cells were surface stained with 0.25 ug Anti-Human HER2/ErbB2 Rabbit RecAb (98304-3-RR, Clone: 242436C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98304-3-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected CHO using 98304-3-RR (same clone as 98304-3-PBS)

  1x10^6 CHO cells or HER2/ErbB2-transfected CHO cells were surface stained with 0.25 ug Anti-Human HER2/ErbB2 Rabbit RecAb (98304-3-RR, Clone: 242436C4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98304-3-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected CHO using 98304-3-RR (same clone as 98304-3-PBS)

  1x10^6 CHO cells or HER2/ErbB2-transfected CHO cells were surface stained with 0.25 ug Anti-Human HER2/ErbB2 Rabbit RecAb (98304-3-RR, Clone: 242436C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98304-3-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98304-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98304-3-RR against Human HER2/ErbB2 were performed. The affinity constant is 3.53 nM.

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Informations sur le produit

98304-3-PBS cible HER2/ErbB2 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Masse moléculaire calculée: 1255 aa, 138 kDa
• Numéro d’acquisition GenBank: BC156755
• Symbole du gène: HER2/ErbB2
• Identification du gène (NCBI): 2064
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

HER2, also known as ErbB2, Neu, and CD340, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.

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