Anticorps Recombinant de lapin anti-HER2/ErbB2

HER2/ErbB2 Recombinant Antibody for FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC

Conjugaison

Non conjugué

CloneNo.

242436C4

N° de cat : 98304-3-RR

Synonymes

ERBB2, CD340, HER 2, HER 2/neu, HER2

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Galerie de données de validation

• 

  FC experiment of MCF-7 using 98304-3-RR

  1x10^6 MCF-7 cells were surface stained with 0.25 ug Anti-Human HER2/ErbB2 Rabbit RecAb (98304-3-RR, Clone: 242436C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected CHO using 98304-3-RR

  1x10^6 CHO cells or HER2/ErbB2-transfected CHO cells were surface stained with 0.25 ug Anti-Human HER2/ErbB2 Rabbit RecAb (98304-3-RR, Clone: 242436C4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected CHO using 98304-3-RR

  1x10^6 CHO cells or HER2/ErbB2-transfected CHO cells were surface stained with 0.25 ug Anti-Human HER2/ErbB2 Rabbit RecAb (98304-3-RR, Clone: 242436C4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98304-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98304-3-RR against Human HER2/ErbB2 were performed. The affinity constant is 3.53 nM.

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Applications testées

• Résultats positifs en cytométrie: cellules MCF-7, Transfected CHO cells

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98304-3-RR cible HER2/ErbB2 dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Masse moléculaire calculée: 1255 aa, 138 kDa
• Numéro d’acquisition GenBank: BC156755
• Symbole du gène: HER2/ErbB2
• Identification du gène (NCBI): 2064
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

HER2, also known as ErbB2, Neu, and CD340, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.

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