Anticorps Polyclonal de lapin anti-HERC2

HERC2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27459-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 27459-1-AP (same clone as 27459-1-PBS)

  HeLa cell lysates were subjected to SDS PAGE followed by western blot with 27459-1-AP (HERC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27459-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 27459-1-AP (same clone as 27459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27459-1-AP (HERC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27459-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 27459-1-AP (same clone as 27459-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27459-1-AP (HERC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27459-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 27459-1-AP (same clone as 27459-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HERC2 antibody (27459-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27459-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27459-1-PBS cible HERC2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HERC2 Protéine recombinante Ag26501
• Nom complet: hect domain and RLD 2
• Masse moléculaire calculée: 527 kDa
• Poids moléculaire observé: 527 kDa
• Numéro d’acquisition GenBank: BC047911
• Symbole du gène: HERC2
• Identification du gène (NCBI): 8924
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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